Selective Isolation of N-Blocked Peptides by Isocyanate-Coupled Resin
Authors:
- 14 September 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 79 (20) , 7910-7915
Abstract
We developed a method for selective isolation of N-blocked peptides from a complex mixture such as an enzymatic digest of a protein. The approach is based on a newly designed isocyanate-resin (resin−NCO), which specifically reacts with α-amino or imino groups. This method, then, permits the isolation of N-blocked peptides, even those containing Lys, from a peptide mixture as intact forms by trapping N-free peptides via covalent bonding to the resin−NCO. The present study demonstrates the performance of this method for the selective isolation of N-blocked peptides by applying it to several peptide mixtures, including proteolytic digests.Keywords
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