Comparative effects of high glucose on different adult sympathetic neurons in culture
Authors:
- Neil C. E. SmithNSNeil C. E. Smith
- Jill LincolnJLJill Lincoln
- 1 October 2004
- journal article
- research article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 15 (15) , 2321-2325
Abstract
In vivo, diabetes causes neuropathy in the sympathetic celiac/superior mesenteric ganglion (CG/SMG) but not the superior cervical ganglion (SCG). Therefore, our aim was to compare the effects of high glucose in vitro on adult rat SCG and CG/SMG neurons. High glucose decreased viability and neurite outgrowth and increased TUNEL staining in freshly dissociated neurons from both ganglia. In cultures established for 24 h before exposure to high glucose, a significant (p<0.01) decrease in neurite-bearing neurons from CG/SMG but not SCG occurred. CG/SMG neurons took longer (p<0.05) to initiate neurite outgrowth and had lower (p<0.002) anti-oxidant defence enzyme activity. These properties may contribute to the selective development of neuropathy in CG/SMG in diabetes in vivo.Keywords
This publication has 22 references indexed in Scilit:
- Activating transcription factor 3 mRNA is upregulated in primary cultures of trigeminal ganglion neuronsMolecular Brain Research, 2003
- Diabetic neuropathy and nerve regenerationProgress in Neurobiology, 2003
- Neuropathology and pathogenesis of diabetic autonomic neuropathy2002
- A role for mitogen‐activated protein kinases in the etiology of diabetic neuropathyThe FASEB Journal, 2001
- Serum-free culture of dissociated, purified adult and aged sympathetic neurons and quantitative assays of growth and survivalJournal of Neuroscience Methods, 2001
- Neurons Undergo Apoptosis in Animal and Cell Culture Models of DiabetesNeurobiology of Disease, 1999
- Insulin-Like Growth Factor-I Prevents Apoptosis in Sympathetic Neurons Exposed to High GlucoseHormone and Metabolic Research, 1999
- The Aetiology of Diabetic Neuropathy: the Combined Roles of Metabolic and Vascular DefectsDiabetic Medicine, 1995
- Differential effect of streptozotocin-induced diabetes on the innervation of the ileum and distal colonGastroenterology, 1991
- Diabetic Autonomic Neuropathy: Present Insights and Future ProspectsDiabetes Care, 1986