A plant Lysin Motif Receptor-Like Kinase plays an ancestral function in mycorrhiza

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Abstract
Arbuscular mycorrhiza (AM) with soilborne Glomeromycota fungi was pivotal in the conquest of land by plants almost half a billion years ago. In flowering plants, it is hypothesized that AM is initiated by the perception of AM fungi-derived chito- and lipochito-oligosaccharides (COs/LCOs) in the host via Lysin Motif Receptor-Like Kinases (LysM-RLKs). However, it remains uncertain whether plant perception of these molecules is a prerequisite for AM establishment and for its origin. Here, we made use of the reduced LysM-RLK complement present in the liverwort Marchantia paleacea to assess the conservation of the role played by this class of receptors during AM and in CO/LCO perception. Our reverse genetic approach demonstrates the critical function of a single LysM-RLK, MpaLYKa, in AM formation, thereby supporting an ancestral function for this receptor in symbiosis. Binding studies, cytosolic calcium variation recordings and genome-wide transcriptomics indicate that another LysM-RLK of M. paleacea , MpaLYR, is also required for triggering a response to COs and tested LCOs, despite being dispensable for AM formation. Collectively, our results demonstrate that the perception of symbionts by LysM-RLK is an ancestral feature in land plants, and suggest the existence of yet-uncharacterized AM fungi signals.
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Funding Information
  • Bill and Melinda Gates Foundation (OPP1172165)
  • Foreign, Commonwealth and Development Office (OPP1172165)
  • EC | ERC | HORIZON EUROPE European Research Council (101001675)
  • LABoratoires d'EXcellence ARCANE (ANR-17-EURE-0003)
  • Glyc@Alps (ANR-15-IDEX-02)
  • Institut Carnot PolyNat (CARN-025-01)
  • Laboratoire d'Excellence TULIP (ANR-10-LABX-41)
  • École Universitaire de Recherche (EUR) - TULIP-GS (ANR-18-EURE-0019)

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