A Comprehensive NMR Analysis of Serum and Fecal Metabolites in Familial Dysautonomia Patients Reveals Significant Metabolic Perturbations

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Abstract
Central metabolism has a profound impact on the clinical phenotypes and penetrance of neurological diseases such as Alzheimer’s (AD) and Parkinson’s (PD) diseases, Amyotrophic Lateral Sclerosis (ALS) and Autism Spectrum Disorder (ASD). In contrast to the multifactorial origin of these neurological diseases, neurodevelopmental impairment and neurodegeneration in Familial Dysautonomia (FD) results from a single point mutation in the ELP1 gene. FD patients represent a well-defined population who can help us better understand the cellular networks underlying neurodegeneration, and how disease traits are affected by metabolic dysfunction, which in turn may contribute to dysregulation of the gut–brain axis of FD. Here, 1H NMR spectroscopy was employed to characterize the serum and fecal metabolomes of FD patients, and to assess similarities and differences in the polar metabolite profiles between FD patients and healthy relative controls. Findings from this work revealed noteworthy metabolic alterations reflected in energy (ATP) production, mitochondrial function, amino acid and nucleotide catabolism, neurosignaling molecules, and gut-microbial metabolism. These results provide further evidence for a close interconnection between metabolism, neurodegeneration, and gut microbiome dysbiosis in FD, and create an opportunity to explore whether metabolic interventions targeting the gut–brain–metabolism axis of FD could be used to redress or slow down the progressive neurodegeneration observed in FD patients.
Funding Information
  • National Institutes of Health (R01-DK117473, 3R01DK117473-02S1, P20GM-103474, 1S10RR13878, 1S10RR026659, NSF-MRI-DBI-1532078, NSF-MRI-CHE-2018388, 2015066:MNL)
  • Alfred P. Sloan Foundation Program (R01-DK117473, 3R01DK117473-02S1, P20GM-103474, 1S10RR13878, 1S10RR026659, NSF-MRI-DBI-1532078, NSF-MRI-CHE-2018388, 2015066:MNL)
  • the Montana INBRE Native American Graduate Fellowship (R01-DK117473, 3R01DK117473-02S1, P20GM-103474, 1S10RR13878, 1S10RR026659, NSF-MRI-DBI-1532078, NSF-MRI-CHE-2018388, 2015066:MNL)
  • Molecular Biosciences Program from Montana State University (MSU) (R01-DK117473, 3R01DK117473-02S1, P20GM-103474, 1S10RR13878, 1S10RR026659, NSF-MRI-DBI-1532078, NSF-MRI-CHE-2018388, 2015066:MNL)
  • NIH Shared Instrumentation Grant (SIG) program (R01-DK117473, 3R01DK117473-02S1, P20GM-103474, 1S10RR13878, 1S10RR026659, NSF-MRI-DBI-1532078, NSF-MRI-CHE-2018388, 2015066:MNL)
  • National Science Foundation (R01-DK117473, 3R01DK117473-02S1, P20GM-103474, 1S10RR13878, 1S10RR026659, NSF-MRI-DBI-1532078, NSF-MRI-CHE-2018388, 2015066:MNL)
  • M.J. Murdock Charitable Trust (R01-DK117473, 3R01DK117473-02S1, P20GM-103474, 1S10RR13878, 1S10RR026659, NSF-MRI-DBI-1532078, NSF-MRI-CHE-2018388, 2015066:MNL)
  • MSU’s Office of the Vice President for Research and Economic Development (R01-DK117473, 3R01DK117473-02S1, P20GM-103474, 1S10RR13878, 1S10RR026659, NSF-MRI-DBI-1532078, NSF-MRI-CHE-2018388, 2015066:MNL)

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