Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications
- 3 September 2015
- journal article
- letter
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 87 (19) , 9567-9571
Abstract
A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone was used as a highly efficient composite for electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured redox potential for glucose oxidation was lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity of 42 mA M–1 cm–2, a linear range of glucose detection of 0.5–12 mM, and good reproducibility and stability as well as high selectivity for different interfering compounds. In a semibiofuel cell configuration, the hybrid film generated high power output of 345 μW cm–2. These results demonstrate a promising potential for this composition in various bioelectronic applications.Keywords
Funding Information
- European Research Council (260647)
- Israel Science Foundation (232/13)
- Ministry of Science Infrastructure Research Program in Analytical Chemistry
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