Endonuclease (R) subunits of type‐I and type‐III restriction‐modification enzymes contain a helicase‐like domain
Authors:
- Alexander E. GorbalenyaAlexander E. Gorbalenya
- Eugene V. KooninEKEugene V. Koonin
- 21 October 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 291 (2) , 277-281
Abstract
A statistically significant amino acid sequence similarity is demonstrated between the endonuclease (R) subunit of EcoK restriction‐modification (R‐M) enzyme, and RNA and DNA helicases of the so‐called ‘DEAD’ family. It is further shown that all three known sequences of R subunits of type‐I and type‐III R‐M enzymes contain the conserved amino acid sequence motifs typical of the previously described helicase superfamily II [(1989) Nucleic Acids Res. 17, 4713–4730]. A hypothesis is proposed that these enzymes may exert helicase activity possibly required for local unwinding of DNA in the cleavage sites.Keywords
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