ENZYMATIC CHARACTERIZATION OF 17l-ARABINOSE NEGATIVE MUTANTS OFESCHERICHIA COLI
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Open Access
- 1 June 1961
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 81 (6) , 996-1006
Abstract
The linear order of 17 closely linked L-arabinose negative (nonutilizing) mutant sites on the genetic map of Escherichia coli strain B/r has been established by three factor transduction experiments, and was found to correspond completely with the organization of these mutants into three functional groups (A, B, and C) on the basis of growth and accumulation studies (Gross and Englesberg, 1959). In this paper the details of growth and accumulation studies and an enzymatic analysis of the mutants shall be presented. MATERIALS AND METHODS Mlledia and strains. The media, arabinose negative mutants (ara-1,-2,-3,-4,-5,-6,-7,-8,-12,-13,-14,-15,-16,-19,-21,-23,-24), phage Plbt, phage production, and procedures followed in transduction are as previously described (Gross and Englesberg, 1959). Growth and accumulation experiments. Initial growth and accumulation experiments were conducted in the following manner. The growth from an overnight casein hydrolyzate mineral glucose slant culture was washed off with 1 ml of saline and transferred to a centrifuge cup. AnKeywords
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