High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins
- 29 December 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Inorganic Chemistry
- Vol. 48 (4) , 1278-1280
Abstract
Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated CuII/I reduction potentials relative to the CuII aquo ion. The high potential and low axial hyperfine splitting (CuII electron paramagnetic resonance A∥) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers.Keywords
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