Simple and Rapid Methods for SEM Observation and TEM Immunolabeling of Rubber Particles
Open Access
- 1 August 2003
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 51 (8) , 1105-1108
Abstract
We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde-tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving their integrity.Keywords
This publication has 8 references indexed in Scilit:
- The micromorphology and protein characterization of rubber particles in Ficus carica, Ficus benghalensis and Hevea brasiliensisJournal of Experimental Botany, 2003
- Immuno Electron Microscopic Localization of Peroxidases in the Differentiating Xylem of Populus spp.Holzforschung, 2002
- Microstructure of Purified Rubber ParticlesInternational Journal of Plant Sciences, 2000
- Rubber particles from four different species, examined by transmission electron microscopy and electron-paramagnetic-resonance spin labeling, are found to consist of a homogeneous rubber core enclosed by a contiguous, monolayer biomembranePlanta, 1999
- Isolation, Characterization, and Functional Analysis of a Novel cDNA Clone Encoding a Small Rubber Particle Protein from Hevea brasiliensisJournal of Biological Chemistry, 1999
- The Effect of Chemical Fixation and Dehydration on the Preservation of Latex inCalystegia silvatica(Convolvulaceae). Examination of Exudate and Latexin situby Light and Scanning Electron MicroscopyJournal of Experimental Botany, 1989
- The Effect of Tannic Acid on the Preservation of Tissue Culture Cells for Scanning Electron MicroscopyJournal of Electron Microscopy, 1981
- Galloylglucoses of low molecular weight as mordant in electron microscopy. I. Procedure, and evidence for mordanting effect.Journal of Cell Biology, 1976