Bradyrhizobium diazoefficiens USDA 110–Glycine max Interactome Provides Candidate Proteins Associated with Symbiosis

Authors:
Open Access
Publisher website
Google Scholar
Abstract
Although the legume-rhizobium symbiosis is a most important biological process, there is a limited knowledge about the protein interaction network between host and symbiont. Using interolog and domain-based approaches, we constructed an inter-species protein interactome containing 5,115 protein-protein interactions between 2,291 Glycine max and 290 Bradyrhizobium diazoefficiens USDA 110 proteins. The interactome was further validated by expression pattern analysis in nodules, GO term semantic similarity, co-expression analysis and luciferase complementation image assay. In G. max-B. diazoefficiens interactome, bacterial proteins are mainly ion channel and transporters of carbohydrates and cations, while G. max proteins are mainly involved in the processes of metabolism, signal transduction and transport. We also identified the top ten highly interacting proteins (hubs) for each species. KEGG pathway analysis for each hub showed that two 14-3-3 proteins (SGF14g and SGF14k) and five heat shock proteins in G. max are possibly involved in symbiosis, and ten hubs in B. diazoefficiens may be important symbiotic effectors. Subnetwork analysis showed that 18 symbiosis-related SNARE proteins may play roles in regulating bacterial ion channels, and SGF14g and SGF14k possibly regulate the rhizobium dicarboxylate transport protein DctA. The predicted interactome provide a valuable basis for understanding the molecular mechanism of nodulation in soybean.
All Related Versions
Funding Information
  • National Natural Science Foundation of China (31571268)
  • State Key Laboratory of Cotton Biology (CB2017B01)
  • Scientific and Technological Self-innovation Foundation, Huazhong Agricultural University (2014RC020)

This publication has 115 references indexed in Scilit: