The Human LGR7 Low‐Density Lipoprotein Class A Module Requires Calcium for Structure
- 1 May 2005
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1041 (1) , 27-34
Abstract
The relaxin and INSL3 receptors, LGR7 and LGR8, are the only human G-protein-coupled receptors to contain a low-density lipoprotein class-A (LDL-A) module. LDL-A modules are well characterized in a variety of diverse biological functions that involve ligand binding to elicit a response. Common features of the LDL-A modules characterized to date are the conservation of three disulfide bonds and the structural arrangement around a bound calcium ion. In this study we recombinantly produce the human LGR7 LDL-A module for NMR studies and demonstrate that calicum is required for the module to form a stable and correctly folded structure.Keywords
This publication has 22 references indexed in Scilit:
- The Role of a Conserved Acidic Residue in Calcium-dependent Protein Folding for a Low Density Lipoprotein (LDL)-A ModuleJournal of Biological Chemistry, 2004
- Solution Structure of the Viral Receptor Domain of Tva and Its Implications in Viral EntryJournal of Virology, 2002
- Activation of Orphan Receptors by the Hormone RelaxinScience, 2002
- Folding Determinants of LDL Receptor Type A ModulesBiochemistry, 2001
- Role of Calcium in Protein Folding and Function of Tva, the Receptor of Subgroup A Avian Sarcoma and Leukosis VirusJournal of Virology, 2001
- Solution Structure of the Sixth LDL-A Module of the LDL Receptor,Biochemistry, 2000
- NMRPipe: A multidimensional spectral processing system based on UNIX pipesJournal of Biomolecular NMR, 1995
- Three-Dimensional Structure of the Second Cysteine-Rich Repeat from the Human Low-Density Lipoprotein ReceptorBiochemistry, 1995
- A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptorCell, 1993
- A Receptor-Mediated Pathway for Cholesterol HomeostasisScience, 1986