Backbone 1H, 15N and 13C resonance assignments of the 27kDa fluorescent protein mCherry
Open Access
- 9 September 2023
- journal article
- research article
- Published by Springer Nature in Biomolecular NMR Assignments
- Vol. 17 (2) , 243-247
Abstract
MCherry is one of the most successfully applied monomeric red fluorescent proteins (RFPs) for in vivo and in vitro imaging. However, questions pertaining to the photostability of the RFPs remain and rational further engineering of their photostability requires information about the fluorescence quenching mechanism in solution. To this end, NMR spectroscopic investigations might be helpful, and we present the near-complete backbone NMR chemical shift assignment to aid in this pursuit.Keywords
Funding Information
- Johannes Kepler University Linz
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