Clonogenic hepatoblasts, common precursors for hepatocytic and biliary lineages, are lacking classical major histocompatibility complex class I antigen

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Abstract
Anin vitrocolony-forming assay and flow cytometry were used to identify rat hepatoblasts as being classical MHC class I, RT1Al-, OX18lowintercellular adhesion molecule 1 (ICAM-1)+. Inducible differentiation toward biliary lineage was observed in most colonies derived from single RT1Al-progenitors, proving their bipotentiality. These findings demonstrate the antigenic profile of clonogenic hepatoblasts and proof of their bipotency. Furthermore, whereas colony formation of adult hepatocytes required epidermal growth factor, clonal growth of hepatoblasts was potentiated without epidermal growth factor. The adult hepatic colonies consisted of RT1Al+OX18+ICAM-1++cells. These results indicate that hepatoblasts possess unique characteristics as compared with adult hepatocytes harboring significant proliferative activity. The phenotypic identity of hepatoblasts and the clonal culture system have relevance for identifying hepatic stem cells from adults, for studying liver development, and for cell therapy based on hepatic progenitors.

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