Blocking of HLA and B Lymphocyte Alloantigens with F(ab′)2 Fragments of Rabbit Antibodies

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Abstract
Summary: F(ab′)2 fragments of rabbit antibodies specific for human B lymphocytes will block human anti-B lymphocyte alloantibodies. F(ab′)2 of anti-β2m will block HLA alloantibodies. The F(ab′)2 fragments can be used to improve the typing of B cell lines and certain leukemia cells which express both HLA and B lymphocyte alloantigens. Pretreatment of these cells with anti-β2m F(ab′)2 preparations allows the observation of B cell alloantigen reactions independently of HLA reactions. Similar use of rabbit anti-B cell F(ab′)2 allows HLA reactions to be observed free from the “extra reactions” caused by B alloantibodies that are present in many HLA typing sera.