Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins
- 16 February 2009
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 9 (6) , 756-760
Abstract
A proof-of-concept study demonstrated the feasibility of a novel gel-pad microarray on porous silicon chips, by initiation of an atom transfer radical propagation (ATRP) polymerisation of (polyethylene glycol) methacrylate (PEGMA) with surface Si–H species, stepwise chemical conversions of the gel membrane to an NTA-Ni2+/histidine-tagged protein system, and matrix-assisted laser desorption/ionisation mass spectroscopy (MALDI MS) and fluorescence detections.Keywords
This publication has 25 references indexed in Scilit:
- Patterned Biofunctional Poly(acrylic acid) Brushes on Silicon SurfacesBiomacromolecules, 2007
- Multicomponent Polymer BrushesJournal of the American Chemical Society, 2006
- Biofunctionalisation of porous silicon (PS) surfaces by using homobifunctional cross-linkersJournal of Materials Chemistry, 2005
- Reaction of Porous Silicon with Both End-Functionalized Organic Compounds Bearing α-Bromo and ω-Carboxy Groups for Immobilization of BiomoleculesThe Journal of Physical Chemistry B, 2005
- Nitrilotriacetic Acid Functionalized Graft Copolymers: A Polymeric Interface for Selective and Reversible Binding of Histidine‐Tagged ProteinsAdvanced Functional Materials, 2005
- The Quest for High-Speed and Low-Volume BioanalysisAnalytical Chemistry, 2005
- Reactions of Surface Amines with Heterobifunctional Cross-Linkers Bearing Both Succinimidyl Ester and Maleimide for Grafting BiomoleculesThe Journal of Physical Chemistry B, 2004
- Microfluidic biosensing systems : Part I. Development and optimisation of enzymatic chemiluminescent µ-biosensors based on silicon microchipsLab on a Chip, 2004
- Macro-/Nanoporous Silicon as a Support for High-Performance Protein MicroarraysAnalytical Chemistry, 2003
- Enhanced enzyme activity in silicon integrated enzyme reactors utilizing porous silicon as the coupling matrixSensors and Actuators B: Chemical, 1996