DISTORTED2 encodes an ARPC2 subunit of the putative Arabidopsis ARP2/3 complex
Authors:
- Jie LeJLJie Le
- Dipanwita BasuDBDipanwita Basu
- Eileen L. MalleryEMEileen L. Mallery
- Daniel B. SzymanskiDSDaniel B. Szymanski
- 14 April 2004
- journal article
- Published by Wiley in The Plant Journal
- Vol. 38 (3) , 526-538
Abstract
Summary: Arabidopsis trichomes are unicellular, branched structures that have highly constrained requirements for the cytoskeleton. The ‘distorted group’ genes function downstream from microtubule‐based branch initiation, and are required during the actin‐dependent phase of polarized stalk and branch expansion. Of the eight known ‘distorted group’ genes, a subset encode homologs of ARP2/3 complex subunits. In eukaryotic cells, the seven‐protein ARP2/3 complex nucleates actin filament networks that push on the plasma membrane and organelles. In plants cells, the existence and function of an ARP2/3 complex is unclear. In this paper, we report that DISTORTED2 (DIS2) encodes a paralogue of the ARP2/3 complex subunit ARPC2. DIS2 has ARPC2 activity, based on its ability to rescue the growth defects of arpc2 (arc35Δ) null yeast cells. Like known ARPC2s, DIS2 physically interacts with ARPC4. Mutations in DIS2 cause a distorted trichome phenotype, defects in cell–cell adhesion, and a modest reduction in shoot FW. The actin cytoskeleton in dis2 trichomes is extensive, but developing branches fail to generate and maintain highly organized cytoplasmic actin bundles.Keywords
This publication has 51 references indexed in Scilit:
- Mutations in Actin-Related Proteins 2 and 3 Affect Cell Shape Development in ArabidopsisThe Plant Cell, 2003
- Regulation of cell expansion by the DISTORTED genes in Arabidopsis thaliana: actin controls the spatial organization of microtubulesMolecular Genetics and Genomics, 2003
- The Arabidopsis TUBULIN-FOLDING COFACTOR A Gene Is Involved in the Control of the α/β-Tubulin Monomer BalanceThe Plant Cell, 2002
- Remodeling of organelle-bound actin is required for yeast vacuole fusionJournal of Cell Biology, 2002
- The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape Control and Tissue DevelopmentThe Plant Cell, 2002
- Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection fluorescence microscopyProceedings of the National Academy of Sciences, 2001
- Crystal Structure of Arp2/3 ComplexScience, 2001
- A Katanin-like Protein Regulates Normal Cell Wall gBiosynthesis and Cell ElongationThe Plant Cell, 2001
- Interactions among Subunits of Human Arp2/3 Complex: p20-Arc as the HubBiochemical and Biophysical Research Communications, 2001
- SIGNALING TO THE ACTIN CYTOSKELETON IN PLANTSAnnual Review of Plant Biology, 2000