Stability of plasmids in five strains of Salmonella maintained in stab culture at different temperatures
Open Access
- 1 August 1994
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 77 (2) , 155-159
Abstract
Four strains of Salmonella berta and one of Salm. enteritidis were stored as stab cultures in sugar‐free agar at 5°, 22° and 30°C and in 15% glycerol at—80°C. The stability of the plasmid profiles in each of the strains was monitored over a period of 2·5 years. Plasmid profiles were stable in all strains stored at—80°C, and only six of 450 colonies examined from strains kept in sugar‐free agar at 5°C had lost plasmid molecules. Seventy of 440 colonies from stab cultures that were kept at 22°C, and 71 of 440 colonies at 30°C showed changed plasmid profiles. The total number of plasmids lost increased with time, and occasionally, more than one plasmid molecule was lost in the same strain. The virulence associated plasmid of Salm. enteritidis was remarkably stable as it was maintained in all colonies examined at all temperatures investigated. Likewise, no change in Sma I restriction profile was observed in this plasmid molecule at any temperature.Keywords
This publication has 18 references indexed in Scilit:
- Molecular characterization of plasmids in Salmonella enteritidis phage typesEpidemiology and Infection, 1993
- Salmonella enterica: Infection, Cross Infection and Persistence within the Environment of a Broiler Parent Stock Unit in DenmarkZentralblatt für Bakteriologie, 1992
- Salmonella plasmids of the pre-antibiotic eraJournal of General Microbiology, 1992
- Plasmid profiles as an epidemiological marker in Salmonella enterica serovar berta infectionsAPMIS, 1992
- Plasmid profiles of Salmonella enterica serovar berta isolated from broilers in DenmarkAPMIS, 1991
- The prevalence of plasmids in Danish bovine and human isolates of Salmonella dublinAPMIS, 1990
- An improved method for rapid isolation of plasmid DNA from wild-type Gram-negative bacteria for plasmid restriction profile analysisLetters in Applied Microbiology, 1990
- Virulence ofSalmonella enteritidisphage type 4 is related to the possession of a 38 MDa plasmidFEMS Microbiology Letters, 1989
- Investigation of the Effect of Growth Environment on the Stability of Low-copy-number Plasmids in Escherichia coli.Microbiology, 1987
- A multiple plasmid-containing Escherichia coli strain: Convenient source of size reference plasmid moleculesPlasmid, 1978