Evaluation of the protective effects of α-tocopherol and retinol against ochratoxin A cytotoxicity
Authors:
- A. BaldiABA. Baldi
- M. N. LosioMLM. N. Losio
- F. CheliFCF. Cheli
- R. RebucciRRR. Rebucci
- L. SangalliLSL. Sangalli
- E. FusiEFE. Fusi
- B. BertasiBBB. Bertasi
- E. PavoniEPE. Pavoni
- S. CarliSCS. Carli
- 1 April 2004
- journal article
- Published by Cambridge University Press (CUP) in British Journal of Nutrition
- Vol. 91 (4) , 507-512
Abstract
Ochratoxin A (OTA), a mycotoxin frequently present in food and feedstuffs, produces a wide range of toxic effects, including cell death via lipid peroxidation. In one human and four animal cell lines we determined the half lethal concentration (LC50) of OTA, its effect on reactive oxygen species (ROS) production, and its ability to induce cytochrome p450 activity. We also examined the protective effect of α-tocopherol and all-trans-retinol in the most sensitive cell lines (i.e. bovine mammary epithelia, for which LC50was 0·8μg/ml (24h), and Madin Darby canine kidney, for which LC50was 4·3μg/ml (48h)). Pre-incubation for 3h with either antioxidant significantly (PP<0·05) ROS production. These findings indicate that oxidative stress is an important factor in OTA cytotoxicity. Supplementation with antioxidant molecules may counteract the short-term toxicity of this mycotoxin.Keywords
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