Cyclin B1/Cdk1 binds and phosphorylates Filamin A and regulates its ability to cross‐link actin
Authors:
- Yun LiYLYun Li
- Jonathan M. LeeJLJonathan M. Lee
- 28 March 2007
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (8) , 1661-1672
Abstract
Substantial actin remodelling occurs prior to mitosis as cells alter their shape in preparation for cytokinesis. In mammalian cells, mitosis is initiated by a heterodimer of cyclin B1 and the cyclin dependent kinase 1 (Cdk1) serine/threonine kinase. In this report. we show that human cyclin B1 binds the actin cross‐linking protein Filamin‐A (FLNa). The proteins co‐immunoprecipitate and co‐localize in mitotic human cells. We find that cyclin B1/Cdk1 can phosphorylate FLNa in vitro and reduce its ability to gelate actin. We have also identified serine 1436 as one FLNa residue phosphorylated by cyclin B1/Cdk1 in vitro. Our results suggest a role for cyclin B1/Cdk1 in FLNa‐dependent actin remodelling.Keywords
This publication has 44 references indexed in Scilit:
- Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-ArrestinsMolecular and Cellular Biology, 2006
- The F-actin Cross-linking and Focal Adhesion Protein Filamin A Is a Ligand and in Vivo Substrate for Protein Kinase CαJournal of Biological Chemistry, 2003
- Roscovitine and Other Purines as Kinase Inhibitors. From Starfish Oocytes to Clinical TrialsAccounts of Chemical Research, 2003
- Regulation of Peroxiredoxin I Activity by Cdc2-mediated PhosphorylationJournal of Biological Chemistry, 2002
- Filamin (280-kDa Actin-binding Protein) Is a Caspase Substrate and Is Also Cleaved Directly by the Cytotoxic T Lymphocyte Protease Granzyme B during ApoptosisJournal of Biological Chemistry, 2000
- Cytoskeletal Protein ABP-280 Directs the Intracellular Trafficking of Furin and Modulates Proprotein Processing in the Endocytic PathwayJournal of Cell Biology, 1997
- Actin Filament Crosslinking by Chicken Gizzard Filamin Is Regulated by Phosphorylation in VitroBiochemistry, 1995
- Actin-Binding Protein Requirement for Cortical Stability and Efficient LocomotionScience, 1992
- Mitosis‐specific phosphorylation of caldesmon: Possible molecular mechanism of cell rounding during mitosisBioEssays, 1991
- Actin-binding and dimerization domains of HeLa cell filaminBiochemistry, 1988