Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes
Authors:
- 1 April 1981
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 82 (2) , 205-216
Abstract
Fragments from section 3 of the salivary gland X chromosome of D. melanogaster were dissected with a micromanipulator. The DNA was extracted, cut and ligated to a λ vector in a volume of a few nanoliters in an oil chamber monitored through a microscope. From about 10 pg of DNA we obtained 80 recombinant clones, a sample of which were analysed and shown to contain Drosophila DNA which hybridises in situ to the region of section 3 of the X chromosome. With this technique we can isolate clones from any desired region as small as 200 kb from the euchromatic arms of polytene chromosomes.Keywords
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