Isolation and Characterization of Ornithine Decarboxylase Gene from Flounder (Paralichthys olivaceus)
Authors:
- 30 July 2004
- journal article
- research article
- Published by Springer Nature in Marine Biotechnology
- Vol. 6 (5) , 453-462
Abstract
Ornithine decarboxylase (ODC) is a homodimeric enzyme dependent on pyridoxal 5’-phosphate. We identified a complementary DNA clone corresponding to ODC from the brain of adult flounder (Paralichthys olivaceus). The flounder ODC cDNA consisted of 2939 bp encoding 272 amino acid residues. The flounder ODC showed 80.3% sequence identity to zebrafish and 70.8% to rat at the amino acid level. Comparison of the structure and nucleotide sequence of the ODC genes revealed that the gene is highly conserved in the flounder, zebrafish, and rat. The presence of ODC mRNA species in brain, kidney, liver, and embryo was confirmed using the reverse transcriptase polymerase chain reaction. The recombinant protein of flounder ODC containing a short histidine tag at the carboxyl terminus was overexpressed in Escherichia coli BL21 (DE3) codon plus using an inducible T7 expression system, and was purified by Ni-NTA affinity chromatography.Keywords
This publication has 47 references indexed in Scilit:
- Cloning and sequence analysis of the interleukin-8 gene from flounder (Paralichthys olivaceous)Gene, 2001
- Degradation of Ornithine Decarboxylase by the 26S ProteasomeBiochemical and Biophysical Research Communications, 2000
- Crystal structure of human ornithine decarboxylase at 2.1 å resolution: structural insights to antizyme bindingJournal of Molecular Biology, 2000
- Recombinant brain 4-aminobutyrate aminotransferases overexpression, purification, and identification of Lys-330 at the active siteBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1997
- Domain Organization and a Protease-Sensitive Loop in Eukaryotic Ornithine DecarboxylaseBiochemistry, 1995
- Molecular cloning of a bovine ornithine decarboxylase cDNA and its use in the detection of restriction fragment length polymorphisms in HolsteinsGenome, 1995
- TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environmentBioinformatics, 1994
- Isolation and Characterization of the Drosophila Ornithine Decarboxylase Locus: Evidence for the Presence of Two Transcribed ODC Genes in the Drosophila GenomeDNA and Cell Biology, 1993
- Feedback regulation of polyamine synthesis in Ehrlich ascites tumor cells. Analysis using nonmetabolizable derivatives of putrescine and spormineBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1988
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970