Metabolite-enhanced normothermic machine perfusion improves kidney transplant viability
Authors:
- Jan CzogallaJCJan Czogalla
- Fabian HausmannFHFabian Hausmann
- Simon LagiesSLSimon Lagies
- Sydney E. GiesSGSydney E. Gies
- Sabrina ChristiansenSCSabrina Christiansen
- Nico KaiserNKNico Kaiser
- Fabian HaasFHFabian Haas
- Yusuke OkabayashiYOYusuke Okabayashi
- Dominik KyliesDKDominik Kylies
- Smilla HofmannSHSmilla Hofmann
- Rossana FranzinRFRossana Franzin
- Niklas SabraNSNiklas Sabra
Open Access
- 23 September 2025
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 10 (20)
Abstract
Normothermic machine perfusion (NMP) has become a valuable tool to expand the pool of transplantable organs. However, the application of NMP to kidneys presents substantial challenges, mostly due to high variability in the composition of currently used perfusion solutions. Here, we provide a multimodal cross-species cellular atlas of kidney injury associated with NMP using a literature-based consensus buffer. This resource provided a systematic framework that was used to develop a metabolite-enhanced perfusion solution, which protected renal proximal tubular cells, improving cellular viability and transplantation outcomes across species, including human kidneys.Keywords
Funding Information
- Deutsche Forschungsgemeinschaft (SFB 1192)
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