Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces

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Abstract
Protein‐based nanoparticles hold promise for a broad range of applications. Here, we report the production of a uniform anionic hollow protein nanoparticle, designated TIP60, which spontaneously assembles from a designed fusion protein subunit based on the geometric features of polyhedra. We show that TIP60 tolerates mutation and both its interior and exterior surfaces can be chemically modified. Moreover, TIP60 forms larger structures upon the addition of a cationic protein. Therefore, TIP60 can be used as a modifiable nano‐building block for further molecular assembly.
Funding Information
  • Japan Society for the Promotion of Science (JP18K05324, JP16H00761, JP16K05841)
  • Keio University
  • Institute for Molecular Science

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