Expression of human angiogenin in cultured baby hamster kidney cells

Authors:
  • SRSusanna M. Rybak
    Susanna M. Rybak
  • JFJames W. Fett
    James W. Fett
  • RSRobert Shapiro
    Robert Shapiro
  • DSDaniel J. Strydom
    Daniel J. Strydom
  • KOKaren A. Olson
    Karen A. Olson
  • JRJames F. Riordan
    James F. Riordan
  • EDEarl W. Davie
    Earl W. Davie
  • BVBert L. Vallee
    Bert L. Vallee
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Abstract
Baby hamster kidney cells were transformed with DNA sequences derived from the gene for human angiogenin. Expression was under the transcriptional control of the inducible mouse metallothionein 1 promoter. Recombinant angiogenin was purified and shown to be chemically, biologically, and enzymatically indistinguishable from the natural product. The large-scale production of recombinant angiogenin achieved should facilitate detailed studies into the structure-function relationships of this potent angiogenic molecule.