Melanin biosynthesis: a study of polyphenol deoxygenation
Authors:
- Fabrice VivianiFVFabrice Viviani
- Michel GaudryMGMichel Gaudry
- Andrée MarquetAMAndrée Marquet
- 1 January 1990
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 1
- No. 5,p. 1255-1259
Abstract
The 1,3,6,8-tetrahydroxynaphthalene (T4HN) reductase of Verticillium dahliae has been studied in a cell-free system. The use of specifically labelled 4(R)- and [4(S)-2H] NADPH in the reduction of T4HN to scytalone reveals that the label is specifically transferred in the case of [4(S)-2H] NADPH whereas no deuterium transfer occurs with the 4R-isomer. This establishes that the T4HN reductase of V. dahliae is a NADPH-dependent dehydrogenase and that it belongs to class B.Keywords
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