HPLC-Nachweis und Isolierung von Phorbolestern aus Crotonöl

Authors:
  • GTG. Tittel
    G. Tittel
  • HWH. Wagner
    H. Wagner
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Abstract
A HPLC method using reversed-phase columns is described for the determination and isolation of phorbolesters in crotonoil. The identification of the compounds is based on an off-line combination of HPLC-MS, as well as on fatty acid analysis by GC. With this method several new phorbolesters of crotonoil could be detected. This method is also suitable for the determination of phorbol- or similar diterpenesters in other Euphorbiaceae.