The Influence of Metallic Ions on the Autoxidation of Oxymyoglobin

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Abstract
SUMMARY: The autoxidation rate of purified oxymyoglobin is extremely variable. Part of the variability has now been traced to contamination of the oxymyoglobin preparations with metals, particularly copper. Copper added in amounts equimolar to the oxymyoglobin concentration resulted in a 25X increase in the autoxidation rate constant. Iron and zinc were much less active than copper in catalyzing the autoxidation. A variety of conditions known to accelerate the autoxidation of oxymyoglobin were tested to see whether the acceleration was due to contamination with metals.

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