CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci

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Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated that the resulting system, CRISPR/dual-FRET MB, enables dynamic imaging of non-repetitive genomic loci with only three unique sgRNAs.
Funding Information
  • National Key R&D Program of China (2016YFA0100702, 2016YFA0501603)
  • National Natural Science Foundation of China (31771583)

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