miR-CATCH: MicroRNA Capture Affinity Technology
- 23 September 2014
- book chapter
- Published by Springer Nature
- Vol. 1218, 365-373
Abstract
Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.Keywords
This publication has 7 references indexed in Scilit:
- Isolation and identification of cell-specific microRNAs targeting a messenger RNA using a biotinylated anti-sense oligonucleotide capture affinity techniqueNucleic Acids Research, 2013
- miR‐TRAP: A Benchtop Chemical Biology Strategy to Identify microRNA TargetsAngewandte Chemie, 2012
- Transcriptome-wide Identification of RNA-Binding Protein and MicroRNA Target Sites by PAR-CLIPCell, 2010
- Tandem affinity purification of miRNA target mRNAs (TAP-Tar)Nucleic Acids Research, 2009
- Argonaute HITS-CLIP decodes microRNA–mRNA interaction mapsNature, 2009
- Switching from Repression to Activation: MicroRNAs Can Up-Regulate TranslationScience, 2007
- Mfold web server for nucleic acid folding and hybridization predictionNucleic Acids Research, 2003