Carnoy's fixative reduces the number of chymase‐positive cells in immunocytochemical staining of cord‐blood‐derived human cultured mast cells
Authors:
Abstract
KleinJan et al, (Allergy 1996;51:614‐20) reported that Carnoy's fixative reduced the number of chymase‐positive mast cells in the nasal mucosa. Therefore, in the present study, we investigated whether Carnoy's fixative reduces the number of chymase‐positive cells from cord‐blood‐derived human cultured mast cells when compared with other types of fixatives. Human mast cells were obtained by culturing cord‐blood‐derived CD34‐positive cells in the presence of stem cell factor and interleukin‐6. Staining procedures of the cells in fixation with Carnoy's fixative and with other fixatives gave no differences among the number of tryptase‐positive cells, whereas fixation with Carnoy's fixative for 15 min gave a significant decrease i n the number of chymase‐positive cells compared with acetone for 10 min. The number of chymase‐positive cells decreased in a time‐dependent manner under fixation with Carnoy's fixative, indicating that Carnoy's fixative had a negative effect on the number of chymase‐positive cells from cord‐blood derived human cultured mast cells.Keywords
This publication has 10 references indexed in Scilit:
- Fixation with Carnoy's fluid reduces the number of chymase-positive mast cells: not all chymase-positive mast cells are also positive for tryptaseAllergy, 1996
- Selective growth of human mast cells induced by Steel factor, IL-6, and prostaglandin E2 from cord blood mononuclear cellsThe Journal of Immunology, 1996
- Characteristics of histamine release from cultured human mast cellsClinical & Experimental Allergy, 1996
- Effects of T-helper 2-type cytokines, interleukin-3 (IL-3), IL-4, IL-5, and IL-6 on the survival of cultured human mast cellsBlood, 1995
- Characterization of Cord-Blood-Derived Human Mast Cells Cultured in the Presence of Steel Factor and lnterleukin-6International Archives of Allergy and Immunology, 1995
- Detection of MCT and MCTC types of human mast cells by immunohistochemistry using new monoclonal anti-tryptase and anti-chymase antibodies.Journal of Histochemistry & Cytochemistry, 1989
- Two types of human mast cells that have distinct neutral protease compositions.Proceedings of the National Academy of Sciences, 1986
- Heterogeneity of mammalian mast cells differentiated in vivo and in vitroJournal of Allergy and Clinical Immunology, 1985
- Comparative Aspects of Mast Cell Heterogeneity in Different Species and SitesInternational Archives of Allergy and Immunology, 1985
- MAST CELLS IN RAT GASTROINTESTINAL MUCOSAActa Pathologica Microbiologica Scandinavica, 1966