Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus

Authors:
  • YNYuxin Ni
    Yuxin Ni
  • AHAtsuko Hata
    Atsuko Hata
  • MYMyonsun Yoh
    Myonsun Yoh
  • TMToshio Miwatani
    Toshio Miwatani
  • TOTadashi Okamoto
    Tadashi Okamoto
  • KGKiyota Goshima
    Kiyota Goshima
  • HTHikaru Takakura
    Hikaru Takakura
  • STSusumu Tsunasawa
    Susumu Tsunasawa
  • FSFumio Sakiyama
    Fumio Sakiyama
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Abstract
A hemolytic toxin (Vp-TRH) produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus was further characterized. The purified Vp-TRH showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (Vp-TDH), a pathogenic toxin produced by Kanagawa phenomenon positive V. parahaemolyticus. Immunological similarities of Vp-TRH not only to Vp-TDH but also to hemolytic toxins produced by Vibrio hollisae and Vibrio cholerae non-O1, both of which are also enteropathogens closely related to V. parahaemolyticus, were demonstrated. The amino acid composition and sequence of N-terminal amino acids of Vp-TRH were determined. These results suggest that Vp-TRH has biological and immunological characters similar to Vp-TDH, although they are distinct molecules. Key words: thermostable direct hemolysin, Vibrio parahaemolyticus, Kanagawa phenomenon.

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