Enhanced Secretion of Prostaglandin E2 by Tissue-Fixed Macrophages in Colonic Carcinoma

Authors:
  • DKD. Kelleher
    D. Kelleher
  • JKJ.J. Keating
    J.J. Keating
  • FHF.P. Hogan
    F.P. Hogan
  • FBF.J. Bloomfield
    F.J. Bloomfield
  • GMG.S. MacDonald
    G.S. MacDonald
  • PKP.W.N. Keeling
    P.W.N. Keeling
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Abstract
Prostaglandin E2 (PGE2) secretion by peripheral blood and tissue-fixed macrophages from patients with colorectal carcinoma was assessed. There was no significant difference between PGE2 production by peripheral blood mononuclear cells between patients with colorectal carcinoma and normal controls. However, secretion of PGE2 by tissue-fixed macrophages from within the colorectal carcinomata in response to opsonised zymosan was significantly higher than in the uninvolved colonic tissue. PGE2 production by tissue-fixed macrophages from within colonic polyps was found to be normal. These results could not be explained on the basis of increased availability of substrate arachidonic acid since addition of excess arachidonic acid resulted in similar findings. The enhanced production of PGE2 correlated with Dukes staging but not the level of differentiation. The production of PGE2 from epithelial cells in response to ionophore A23187 was not significantly enhanced. Leukotriene B4 secretion by intestinal macrophages in response to opsonised zymosan was not significantly elevated in the colonic tumour tissue. Modulation of levels of prostaglandin production within colonic tumours may play a role in the rate of growth and vascularity of these tumours and in the regulation of the local immune response to malignancy.