Development of a Rapid ATP Bioluminescence Assay for Biocidal Susceptibility Testing of Rapidly Growing Mycobacteria
Authors:
- 1 October 2010
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 48 (10) , 3725-3728
Abstract
An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The test species, M ycobacterium immunogenum , M. chelonae , and M. abscessus , showed various susceptibilities to the glutaraldehyde- and isothiazolone-based test biocides.Keywords
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