Fractionation of linseed phosphatides

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Abstract
Summary: Alcohol‐soluble and alcohol‐insoluble fractions of linseed phosphatides were subjected to countercurrent distribution in the Craig apparatus. The soluble portion, which was shown by analysis to contain essentially all of the choline and very little of the inositol, gave little further fractionation on distribution between hexane and 90% methanol. Distribution of the alcohol‐insoluble phosphatides between hexane and 90% ethanol showed two types of phosphoinositides to be present. Those concentrated in the alcohol phase had a phosphorus:nitrogen:inositol ratio of approximately 2:1:1 whereas those more soluble in hexane had a ratio of approximately 4:4:1.Analysis for “ethanolamine” by the periodate method failed to show as much amino nitrogen as analysis by the Van Slyke method. This difference, considered with the shape of the ethanolamine nitrogen curves, prevents a satisfactory calculation of the amount of cephalin.Sugar occurs also in all fractions, but its mode of combination, if any, was not demonstrated.

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