The small chemical vacuolin‐1 alters the morphology of lysosomes without inhibiting Ca2+‐regulated exocytosis

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Abstract
Ca2+‐regulated exocytosis of lysosomes was previously shown to be required for the repair of plasma membrane wounds. The small chemical vacuolin‐1 alters the morphology of lysosomes without affecting the ability of cells to reseal their plasma membrane after injury. On the basis of a failure to detect Ca2+‐triggered lysosomal exocytosis in vacuolin‐1‐treated cells, a recent study proposed that lysosomes are dispensable for resealing. Here, we show that vacuolin‐1, despite altering lysosome morphology, does not inhibit the exocytosis of lysosomes induced by exposure to a Ca2+ ionophore, or by plasma membrane wounding. Thus, lysosomes cannot be excluded as agents of membrane repair in vacuolin‐1‐treated cells.

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