MICROMETHOD OF BLOOD DIAZEPAM DETERMINATION BY GAZ LIQUID-CHROMATOGRAPHY APPLICATION IN NEONATAL PHARMACOLOGY

Authors:
  • NSN Scagliola
    N Scagliola
  • RJR Julien
    R Julien
  • CDC Dreux
    C Dreux
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  • 1 January 1976
    • journal article
    • research article
    • Vol. 34  (1) , 27-32
    • PMID:970696
Abstract
A micromethod of diazepam determination of 0.1 ml of whole blood is proposed. The extraction is made with n-heptane for the isolation of diazepam in the presence of principal metabolites. For the usual concentrations, the precision is about 3%; at the detection limit, 0.05 .mu.g/ml, it is about 20%. With this method the pharmacokinetics of diazepam were studied in [human] neonates with convulsive diseases and compared with ECG results.