Heme Oxygenase 1 in Cerebrospinal Fluid from Infants and Children after Severe Traumatic Brain Injury
Authors:
- Yichen LaiYichen Lai
- Christina D. MarcoCMChristina D. Marco
- Hülya BayırHBHülya Bayır
- P. David AdelsonPAP. David Adelson
- Keri L. Janesko-FeldmanKJKeri L. Janesko-Feldman
- Patrick M. KochanekPKPatrick M. Kochanek
- Robert S.B. ClarkRCRobert S.B. Clark
- 1 August 2006
- journal article
- research article
- Published by S. Karger AG in Developmental Neuroscience
- Vol. 28 (4-5) , 342-347
Abstract
Heme oxygenase 1 (HO-1) is an enzyme important in the catabolism of heme that is induced under conditions of oxidative stress. HO-1 degradation of heme yields biliverdin, bilirubin, carbon monoxide and iron. HO-1 is thought to serve a protective antioxidant function, and upregulation of HO-1 has been demonstrated in experimental models of neurodegeneration, subarachnoid hemorrhage, cerebral ischemia and traumatic brain injury (TBI). We measured HO-1 concentration in cerebral spinal fluid samples from 48 infants and children following TBI and 7 control patients by ELISA. Increased HO-1 was seen in TBI versus control patients – mean 2.75 ± 0.63, peak 4.17 ± 0.96 ng/ml versus control (<0.078 ng/ml, not detectable) (p< 0.001). Increased HO-1 concentration was associated with increased injury severity and unfavorable neurological outcome (both p < 0.05). Increased HO-1 concentration was independently associated with younger age; however, statistical analysis could not rule out the possibility that the effect of age was related to inflicted TBI from child abuse. HO-1 increases after TBI and appears to be more prominent in infants compared with older children after injury.Keywords
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