Hydrolysis of feather keratin by immobilized keratinase
Authors:
- X LinXLX Lin
- J ShihJSJ Shih
- H E SwaisgoodHSH E Swaisgood
- 1 November 1996
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 62 (11) , 4273-5
Abstract
Keratinase isolated from Bacillus licheniformis PWD-1 was immobilized on controlled-pore glass beads. The immobilized keratinase demonstrated proteolytic activities against both insoluble feather keratin and soluble casein. It also displayed a higher level of heat stability and an increased tolerance toward acidic pHs compared with the free keratinase. During a continuous reaction at 50(deg)C, the immobilized keratinase retained 40% of the original enzyme activity after 7 days. The immobilized keratinase exhibits improved stability, thereby increasing its potential for use in numerous applications.Keywords
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