Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources
Open Access
- 2 August 2022
- journal article
- research article
- Published by Wiley in Journal of Food Quality
- Vol. 2022, 1-7
Abstract
Proteins play an imperative role in enhancing the nutritional status of the human body. The present study was designed to determine the molecular weight of protein isolates prepared from defatted oilseeds, i.e., sesame, flaxseed, and canola, using SDS-PAGE. The electropherogram revealed protein bands ranging from 15 to 65 kDa. Furthermore, proteins were subjected to amino acid profiling followed by calculation of amino acid score with reference to requirement for preschool children. The amino acid profiling results indicated that sesame protein isolates (SPI) exhibited the highest values for aromatic amino acids, histidine, isoleucine, and valine. However, the maximum values for sulfur-containing amino acids were depicted by flaxseed protein isolates (FPI). Moreover, the lysine content was highest in canola protein isolates (CPI). Results indicated better profile and quality of proteins, capable to meet the requirements of essential amino acids, especially for preschoolers. Moreover, the values for the protein digestibility corrected amino acid score (PDCAAS) and in vitro protein digestibility (IVPD) were also determined. Conclusively, protein isolates from defatted oilseeds exhibit better-quality proteins with a balanced amino acid profile. By potential utilization in numerous food products, these proteins can play a pivotal role in fulfilling the nutritional requirements of individuals, especially in developing economies.Keywords
This publication has 42 references indexed in Scilit:
- Alternative sources of protein for poultry nutrition2017
- Extruded Flaxseed Meal Enhances the Nutritional Quality of Cereal-based ProductsPlant Foods for Human Nutrition, 2013
- A comparative study of physicochemical and conformational properties in three vicilins from Phaseolus legumes: Implications for the structure–function relationshipFood Hydrocolloids, 2011
- Canola proteins: composition, extraction, functional properties, bioactivity, applications as a food ingredient and allergenicity – A practical and critical reviewTrends in Food Science & Technology, 2011
- Effect of differential processing methods on L-Dopa and protein quality in velvet bean, an underutilized pulseLWT, 2008
- Evaluation of mustard (Brassica juncea) protein isolate prepared by steam injection heating for reduction of antinutritional factorsLWT, 2006
- Study of functional properties of seed storage proteins from indigenous European legume crops (lupin, pea, broad bean) in admixture with polysaccharidesFood Hydrocolloids, 2005
- Amino acid composition of three different types of land snails consumed in NigeriaFood Chemistry, 2004
- Protein Digestibility-Corrected Amino Acid Scores for Bean and Bean−Rice Infant Weaning Food ProductsJournal of Agricultural and Food Chemistry, 2001
- A Rapid Protein Digestibility Assay for Identifying Highly Digestible Sorghum LinesCereal Chemistry, 2001