Gas chromatography/combustion/isotope‐ratio‐monitoring mass spectrometric analysis of methylboronic derivatives of monosaccharides: a new method for determining natural 13 C abundances of carbohydrates

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Abstract
Monosaccharides were derivatized using methylboronic acid and N,O‐bis(trimethylsilyl)trifluoroacetamide (BSTFA), and the δ 13 C values of these derivatives measured by gas chromatography/combustion/isotope‐ratio‐monitoring mass spectrometry to determine the original 13 C‐content of the monosaccharides. Comparison with the measured off‐line δ 13 values of the monosaccharides shows that no fractionation in 13 C takes place during derivatization. The methylboronic derivatization method has proven to be a new method for natural abundance isotopic analysis of intact monosaccharides (arabinose, xylose, fucose, fructose and glucose). The method is rapid, does not involve isotopic fractionation during derivatization, and gives more precise δ 13 C values than other methods reported. The method was successfully applied to determine the δ 13 C value of glucose of the freshwater alga Scenedesmus communis . Copyright © 2001 John Wiley & Sons, Ltd.
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  • Netherland-Bremen Oceanography Program

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