Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique.
Authors:
- F G MoserFMF G Moser
- B P DormanBDB P Dorman
- F H RuddleFRF H Ruddle
Open Access
- 1 September 1975
- journal article
- Published by Rockefeller University Press in Journal of Cell Biology
- Vol. 66 (3) , 676-680
Abstract
The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.Keywords
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