A fluorimetric and enzymatic method for the estimation of serum total bile acids
Authors:
- G. M. MurphyGMG. M. Murphy
- Barbara H. BillingBBBarbara H. Billing
- D. N. BaronDBD. N. Baron
Open Access
- 1 October 1970
- journal article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 23 (7) , 594-598
Abstract
The enzymatic technique using 3α-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced patients. The range of normal values in serum was found to be 0-4·7 μmol/litre for males and 1·0-8·2 μmol/litre for females.Keywords
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