A review of the current status of cultural and rapid detection ofVibrio parahaemolyticus

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Summary: Vibrio parahaemolyticusis ubiquitous in estuarine environments and can be commonly found in seafood products. This bacterial pathogen continues to emerge as an important cause of foodborne illness, and several foodborne disease outbreaks caused byV. parahaemolyticushave been linked to the consumption of contaminated seafood, in particular those consumed raw such as oysters. In response to these outbreaks, especially during the 1990s, several cultural, immunological‐based and molecular detection methods have been developed, which allow for rapid detection and quantification of total and pathogenicV. parahaemolyticus. The development of molecular methodology has allowed for clinical and environmental isolates ofV. parahaemolyticusto be subtyped, thus providing the framework for risk‐based strategies aimed at controlling foodborne outbreaks cause by this pathogen. It is important that the detection and typing methods strive to accomplish detection and differentiation of the pathogenic strains from environmental (non‐pathogenic) ones, as well as to detect the presence of the organism and not just the presence ofV. parahaemolyticusproduced toxins, which can also be produced by closely related species. This review covers the current status of detection and typing methodology for identification and characterisation ofV. parahaemolyticusfrom seafood.

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