Separation of anthraquinone compounds from the seed of Cassia obtusifolia L. using recycling counter‐current chromatography
- 4 January 2012
- journal article
- research article
- Published by Wiley in Journal of Separation Science
- Vol. 35 (2) , 256-262
Abstract
Recycling counter-current chromatography (CCC) together with step-gradient CCC and medium-pressure liquid chromatography (MPLC) was employed to separate nine anthraquinone compounds from Cassia obtusifolia L. in this study. The results showed that recycling CCC is a powerful tool for compounds that are difficult to separate with common elution mode. CCC was the better option for crude material while MPLC had advantage for the final tuning. The combination of recycling CCC and MPLC could simplify the method exploring process in the separation process. The structures of these compounds were identified according to their mass spectra, by 1H-NMR and compared with standard compounds.Keywords
Funding Information
- Open-Study Funds of State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine
This publication has 15 references indexed in Scilit:
- Separation and purification of echinacoside from Penstemon barbatus (Can.) Roth by recycling high-speed counter-current chromatographyJournal of Chromatography B, 2010
- Preparative separation and purification of five anthraquinones from Cassia tora L. by high-speed counter-current chromatographySeparation and Purification Technology, 2008
- Flow rate gradient high-speed counter-current chromatography separation of five diterpenoids from Triperygium wilfordii and scale-upJournal of Chromatography A, 2008
- Hypolipidemic activity of seeds of Cassia tora LinnJournal of Ethnopharmacology, 2004
- Cytotoxic Principles from Ventilago leiocarpaJournal of Natural Products, 2001
- Extraction and Identification of an Antioxidative Component from Jue Ming Zi (Cassia tora L.)Journal of Agricultural and Food Chemistry, 1998
- Anthraquinones and a 10-Hydroxyanthrone from Phialophora albaJournal of Natural Products, 1994
- High-Speed Countercurrent ChromatographyCritical Reviews in Analytical Chemistry, 1986
- Studies on the constituents of the seeds of Cassia obtusifolia Linn. The structures of three new anthraquinones.Chemical and Pharmaceutical Bulletin, 1984
- Knipholone: a unique anthraquinone derivative from Kniphofia foliosaPhytochemistry, 1984