Proteolytic Activity of the Roach (Rutilus RutilusL.) Intestinal Microflora

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Abstract
For research, two differently polluted lakes were selected: Lake Dringis, Aukštaitija National Park, as an ecosystem pattern of a weak anthropogenic influence, and Lake Drūkšiai, as a regularly polluted water body. Lake Drūkšiai, the cooling basin of Ignalina Nuclear Power Plant, through its tributaries is being polluted with industrial and municipal sewage. Samples were taken from four differently polluted mouths of tributaries: the mouth of the Ričanka Stream; the mouth of the Gulbinės Stream affected by urban rain sewerage of Visaginas (URS-2); the mouth of the Gulbinėlė Stream into which municipal sewage of Visaginas (MS) and industrial rain sewerage from INPP (IRS-1.2) flow. The work was aimed to determine the role of the symbiotic bacterioflora of the roach digestive tract in protein decomposition, as well as the dependence of proteolytic enzyme activity upon environmental pollution. Comparison of the proteolytic activity (PA) of the roach intestinal bacterioflora showed reliable differences between Lakes Dringis and Drûkðiai. The total mean PA of bacterioflora in Lake Dringis roach intestines was established to be twice higher than that in Drûkðiai. At the URS-2, MS, and IRS-1.2 mouths of Lake Drūkšiai, the mean PAs of the isolates were reliably similar. The mean PA was a little higher in the Ričanka.s mouth roach intestinal bacteria. The highest mean PA in active strains as well as the highest total mean PA was typical of Lake Dringis roach intestinal strains. In Lake Dringis, the highest mean PA in active bacteria strains was determined in the IRS-1.2 mouth roach intestines. The research revealed the enzyme activity in fish intestinal bacterioflora to be dependent upon the ecology state of environment.