Purification, sequencing and synthesis of natriuretic and vasoactive rat atrial peptide

Authors:
  • SAS. A. Atlas
    S. A. Atlas
  • HKH. D. Kleinert
    H. D. Kleinert
  • MCM. J. Camargo
    M. J. Camargo
  • AJA. Januszewicz
    A. Januszewicz
  • JSJ. E. Sealey
    J. E. Sealey
  • JLJ. H. Laragh
    J. H. Laragh
  • JSJ. W. Schilling
    J. W. Schilling
  • JLJ. A. Lewicki
    J. A. Lewicki
  • LJL. K. Johnson
    L. K. Johnson
  • TMT. Maack
    T. Maack
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Abstract
Mammalian atria contain potent natriuretic and diuretic substances which exist in high- and low-molecular-weight forms and which appear to be associated with atrium-specific granules. The natriuretic effect of atrial extract is largely accountable for by its renal haemodynamic effects; atrial extracts also antagonize hormone- and non-hormone-induced contraction of the isolated rabbit aorta and isolated rat kidney vasculature. We have completely purified a low-molecular-weight natriuretic and vasoactive substance from rat atria and characterized it as a 24-amino acid peptide. Synthetic peptide, produced by solid-phase synthesis, mimics biological effects of crude atrial extract and purified peptide; its activity is enhanced by slow oxidation, suggesting a disulphide (Cys 4-Cys 20) configuration for the native peptide. If secreted into blood, this atrial natriuretic peptide (' auriculin B') could be a novel peptide hormone of considerable importance to renal and cardiovascular homeostasis.
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