STIMULATORY EFFECTS OF SUBSTANCE P ON NEURITE EXTENSION AND CYCLIC AMP LEVELS IN CULTURED NEUROBLASTOMA CELLS

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Abstract
Abstract— Synthetic substance P initially increased cyclic AMP levels and subsequently induced neurite extension in cultured neuroblastoma N 18 cells. The magnitude of these effects depended on the concentration of fetal calf serum (FCS) in the culture medium, being more evident in the presence of a lower (0.1%) concentration of FCS.In Eagle's medium containing 0.1% FCS, low concentrations of substance P (10−7‐10−5 M) increased cyclic AMP levels and stimulated neurite extension.In Eagle's medium containing 5%FCS, both substance P at concentrations of 10−5‐10−3M and dibutyryl cyclic AMP at concentrations of 10−4‐10−2M increased cyclic AMP levels and stimulated neurite extension. The activities of acetylcholinesterase, (Na++ K+)‐, HCO3− and Mg2+ ‐stimulated‐ATPase were also increased. Cell growth was inhibited.Substance P at concentrations of 10‐7‐10−5M also stimulated the adenylate cyclase activity of a particulate fraction of N 18 in a concentration‐dependent manner.

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