Effect of Sulfate-Containing Compounds on Methane Production by Using an In vitro Gas Production Technique

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Abstract
The present study demonstrated the effect of levels of sulfate-containing compounds on methane production using in vitro gas production technique. Treatments were TMR without supplement (control) and TMR with 0.2 or 0.4% of ammonium sulfate, copper sulfate and sodium lauryl sulfate in a 3x2 Factorial in CRD with one control. Rumen fluid was collected from two rumen-fistulated beef cattle fed on a based diet. During the incubation, the gas production was recorded at 0, 2, 4, 6, 8, 10, 12, 18, 24, 36, 48, 60, 72 and 96 h. The results revealed that increased the levels of sulfate-containing compounds in the TMR diet decreased the gas accumulation and CH4 accumulation all the time. Although, the sum of the MRP did not differ among treatments, the MRP at 24 h after incubation was greatly appreciated by 0.2% of ammonium sulfate (14.53%). In addition, ammonium sulfate led to the highest IVOMD followed by the copper sulfate. Therefore, this study suggested that ammonium sulfate and copper sulfate and sodium lauryl sulfate at 0.2 and 0.4% in the TMR diet have the potential to reduce methane emission.
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