Essential role of ferrous iron in cyanide‐resistant respiration in Hansenula anomala
Authors:
- 2 July 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 267 (1) , 114-116
Abstract
Antimycin A‐dependent induction of cyanide‐resistant respiration in Hansenula anomala was completely blocked by o‐phenanthroline, α,α'‐dipyridyl. or 8‐hydroxyquinoline. Pulse‐labeling of the cells with [35S] methionine in the presence of both antimycin A and o‐phenanthroline indicated that the 36‐kDa protein previously reported to be involved in cyanide‐resistant respiration [(1989) J. Biochem. 105, 864‐866] was formed in mitochondria even under these conditions. The addition of Fe2+, but not Fe3+, ions to these cells in the presence of cycloheximide resulted in the rapid expression of cyanide‐resistant respiration activity. These results suggest that in the presence of both antimycin A and o‐phenanthroline an inactive form of the 36‐kDa protein was formed and Fe2+ ions converted it to the active form. It is also likely that Fe2+ ions are involved in the reaction mechanism of cyanide‐resistant respiration.Keywords
This publication has 13 references indexed in Scilit:
- Component of the alternative oxidase localized to the matrix surface of the inner membrane of plant mitochondriaFEBS Letters, 1990
- Respiration of Bloodstream Forms of the Parasite Trypanosoma brucei brucei Is Dependent on a Plant-like Alternative OxidaseJournal of Biological Chemistry, 1989
- Immunological identification of the alternative oxidase of Neurospora crassa mitochondria.Molecular and Cellular Biology, 1989
- The induction of cyanide-resistant respiration by carbon monoxide in Hansenula anomala.Agricultural and Biological Chemistry, 1989
- Determination of molecular mass of the aroid alternative oxidase by radiation-inactivation analysisBiochemical Journal, 1988
- Identification of the alternative terminal oxidase of higher plant mitochondriaProceedings of the National Academy of Sciences, 1987
- The Nature of the Terminal Oxidation Step of the Alternative Electron Transport Pathway1987
- The induction of cyanide-resistant respiration in the absence of respiratory inhibitors in Hansenula anomala.Agricultural and Biological Chemistry, 1987
- The Cyanide-Resistant Pathway of Plant Mitochondria1985
- Involvement of iron in the biogenesis of the cyanide-insensitive respiration in the yeast Saccharomycopsis lipolyticaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977