Integrated Pretreatment and Microbial Matching for PHA Production from Lignocellulosic Agro-Forestry Residues

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Abstract
Lignocellulosic agro-forestry residues (LARs), such as rice straw, sugarcane bagasse, and wood wastes, are abundant and low-cost feedstocks for polyhydroxyalkanoate (PHA) bioplastics. However, their complex cellulose–hemicellulose–lignin matrix requires integrated valorization strategies. This review presents a dual-framework approach: “pretreatment–co-substrate compatibility” and “pretreatment–microbial platform matching”, to align advanced pretreatment methods (including deacetylation–microwave integration, deep eutectic solvents, and non-sterilized lignin recovery) with engineered or extremophilic microbial hosts. A “metabolic interaction” perspective on co-substrate fermentation, encompassing dynamic carbon flux allocation, synthetic consortia cooperation, and one-pot process coupling, is used to elevate PHA titers and tailor copolymer composition. In addition, we synthesize comprehensive kinetic analyses from the literature that elucidate microbial growth, substrate consumption, and dynamic carbon flux allocation under feast–famine conditions, thereby informing process optimization and scalability. Microbial platforms are reclassified as broad-substrate, process-compatible, or product-customized categories to emphasize adaptive evolution, CRISPR-guided precision design, and consortia engineering. Finally, next-generation techno-economic analyses, embracing multi-product integration, regional adaptation, and carbon-efficiency metrics, are surveyed to chart viable paths for scaling LAR-to-PHA into circular bioeconomy manufacturing.
Funding Information
  • National Natural Science Foundation of China (32301527)
  • China Postdoctoral Science Foundation (2024M752169)
  • Natural Science Foundation of Tianjin Municipality (23JCZDJC00620)
  • Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education, Qilu University of Technology (KF202209)
  • Natural Science Foundation of Shandong Province (ZR2024MC009)
  • QUTJBZ Program (2024ZDZX01)
  • Taishan Scholars Program of Shandong Province

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